Regulating apoptosis in mammalian cell cultures

Biosafety of Mammalian Cell Cultures

Enzyme activity levels in mammalian cell cultures.

Improved methods for growing animal cells in culture have led to their wide use as a tool in a variety of disciplines in biology. Cultures from many different anatomical origins are available and purification of several of them has been effected by the method of single clone isolation (1). The exact nature of the cultured cells and their relationship to the organ of their origin is, however, un...

Metabolic flux rewiring in mammalian cell cultures.

Continuous cell lines (CCLs) engage in 'wasteful' glucose and glutamine metabolism that leads to accumulation of inhibitory byproducts, primarily lactate and ammonium. Advances in techniques for mapping intracellular carbon fluxes and profiling global changes in enzyme expression have led to a deeper understanding of the molecular drivers underlying these metabolic alterations. However, recent ...

Enzvme Activitv Levels in Mammalian Cell Cultures*

Improved methods for growing animal cells in culture have led to their wide use as a tool in a variety of disciplines in biology. Cultures from many different anatomical origins are available and purification of several of them has been effected by the method of single clone isolation (1). The exact nature of the cultured cells and their relationship to the organ of their origin is, however, un...

Cell cycle and apoptosis in PER.C6® cultures

Background PER.C6 is a human cell line designed for virus production, which was immortalized by transformation with adenoviral E1A and E1B genes. Expression of E1A is known to inhibit negative regulators of cell cycle and E1B protein function analogously to an apoptosis inhibitor. As changes in cell cycle and apoptosis are likely to affect cell’s ability for viral infection and propagation, the...